Cloning of a DNA fragment involved in pigment production in Streptomyces avermitilis
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منابع مشابه
Cloning and analysis of a DNA fragment stimulating avermectin production in various Streptomyces avermitilis strains.
To isolate a gene for stimulating avermectin production, a genomic library of Streptomyces avermitilis ATCC 31267 was constructed in Streptomyces lividans TK21 as the host strain. An 8.0-kb DNA fragment that significantly stimulated actinorhodin and undecylprodigiosin production was isolated. When wild-type S. avermitilis was transformed with the cloned fragment, avermectin production increased...
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The 2015 Nobel Prize in Physiology or Medicine has been awarded to avermectins and artemisinin, respectively. Avermectins produced by Streptomyces avermitilis are excellent anthelmintic and potential antibiotic agents. Because wild-type strains only produce low levels of avermectins, much research effort has focused on improvements in avermectin production to meet the ever increasing demand for...
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I. Biosynthesis of Avermectins A. Incorporation of Labeled Precursors B. Biosynthetically Blocked Mutants and Characteristics of Each Step in Avermectin Biosynthetic Pathway 1. aveA {aveAI and aveAIh step for polyketide synthase) 2. aveB (step for glycosylation) 3. aveC (step for C22~23-dehydration) 4. aveD (step for C5 O-methylation) 5. aveE (step for furan ring formation at C6 to C8a) 6. aveF...
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A DNA fragment from the lignocellulolytic actinomycete Streptomyces avermitilis CECT 3339 was cloned using a DNA probe from the xylanase gene xysA of Streptomyces halstedii. The nucleotide sequence analysis revealed two potential ORFs, xyl30 and hd30, encoding a deduced multimodular F/10 xylanase with a binding domain and a secreted glycoxyl hydrolase, respectively. In Streptomyces lividans car...
متن کاملFunctional expression of SAV3818, a putative TetR-family transcriptional regulatory gene from Streptomyces avermitilis, stimulates antibiotic production in Streptomyces species.
Avermectin and its analogs are major commercial antiparasitic agents in the fields of animal health, agriculture, and human infections. Previously, comparative transcriptome analysis between the low-producer S. avermitilis ATCC31267 and the high-producer S. avermitilis ATCC31780 using a S. avermitilis whole genome chip revealed that 50 genes were overexpressed at least two-fold higher in S. ave...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1992
ISSN: 0378-1097
DOI: 10.1016/0378-1097(92)90555-3